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Caleb Stoltzfus has a long and varied work history. In 2005, they began working as a Shift Manager at Dairy Queen, followed by a role as a Cashier at Town & Country Foods in 2007. In 2010, they began working as an Undergraduate Research Assistant at Montana State University, followed by roles as a Graduate Student, Volunteer Research Assistant, and Senior Fellow. In 2016, they were a Research Scientist at Spectrum Lab. Since 2017, they have been employed at the University of Washington - School of Medicine, where they have held roles as a Washington Research Foundation Postdoctoral Fellow, Research Scientist III, and Senior Fellow. In 2021, they began working as the Director of Research and Development and Principal Engineer at Alpenglow Biosciences.

Caleb Stoltzfus attended Montana State University-Bozeman from 2006 to 2016, earning a Bachelor of Science (BS) in Physics in 2011, a Master of Science (M.S.) in Physics in 2014, and a Doctor of Philosophy (PhD) in Physics in 2016.