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Marie-Isabelle Heiss has a varied work experience. From 2016 to 2017, they were a Rechtsreferendarin at the Auswärtiges Amt (Federal Foreign Office) Germany. During this time, they also held the same role at GvW Graf von Westphalen. In 2017, they were a Public Affairs Manager and Gründungsmitglied & Community Organizer at Volt Europa, where they were responsible for organizational development and capacity development, as well as leading the Kreisverbandes Volt M\u00fcnchen. In 2018, they became a Rechtanw\u00e4ltin at Heiss & Partner. Marie-Isabelle is currently Chief Of Staff and Executive Assistent to the CEO at 1KOMMA5\u00b0, a position they have held since 2022.

Marie-Isabelle Heiss completed their Jurastudium, allgemein at the Universität Passau from 2010 to 2012. Marie-Isabelle then went on to earn their Staatsexamen at the Freie Universität Berlin from 2012 to 2015. Marie-Isabelle is currently pursuing their LL.M. in International Law at the University of Cape Town, which they began in 2020.